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Under construction

MorPhiC gRNA enrichment workflow

Workflow

  • Initial version designed and developed by the Huangfu Lab at Memorial Sloan Kettering Center Institute [1]
  • Main tool for analysis is Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK) [2]

Open in GitPod

Containerized version and documentation are publicly available at https://github.com/morphic-bio/gRNA-Enrichment

References

[1] Rosen BP, Li QV, Cho HS, Liu D et al. Parallel genome-scale CRISPR-Cas9 screens uncouple human pluripotent stem cell identity versus fitness. Nat Commun 2024 Oct 17;15(1):8966. https://pubmed.ncbi.nlm.nih.gov/39419994/

[2] Li, W., Xu, H., Xiao, T. et al. MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens. Genome Biol 15, 554 (2014). https://doi.org/10.1186/s13059-014-0554-4